bovine endothelial cells  (ATCC)

Journal: Food Chemistry: X

Article Title: Nutraceutical potential of radish ( Raphanus sativus cv. Tango) microgreens as sustainable and reproducible sources of hydrogen sulfide-releasing compounds

doi: 10.1016/j.fochx.2026.103816

Figure Lengend Snippet: A) H 2 S measurement from OME (1–1000 μg/mL) in a cell-free assay. Data are reported as μM and expressed as means ± SEM (n = 3; **** p < 0.0001 vs vehicle; °°°° p < 0.0001 vs 100 μg/mL, and §§§§ p < 0.0001 vs 300 μg/mL); B) MTT assay for OME (1–1000 μg/mL) in BAEC C) C) Live fluorometric quantification of H 2 S in BAEC exposed to OME (10–1000 μg/mL) or glucoraphasatin (1–1000 μg/mL). Data are reported as fluorescence intensity and expressed as means ± SEM ( n = 5; ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs vehicle).

Article Snippet: Bovine aortic endothelial cells (BAECs) were purchased from iXCells Biotechnologies (Cat# 10BO-001) and were cultured in Modified Dulbecco's Medium (DMEM) supplemented with 2 mmol/L glutamine, 10% heat-inactivated fetal bovine serum, and 50 U/mL penicillin/streptomycin.

Techniques: Cell-Free Assay, MTT Assay, Fluorescence

Journal: Food Chemistry: X

Article Title: Nutraceutical potential of radish ( Raphanus sativus cv. Tango) microgreens as sustainable and reproducible sources of hydrogen sulfide-releasing compounds

doi: 10.1016/j.fochx.2026.103816

Figure Lengend Snippet: A) NO measurement ( n = 4; ** p < 0.01 vs vehicle, ° p < 0.05 vs NaP) and B) ROS measurement (*** p < 0.001 vs vehicle, ° p < 0.05 vs NaP) in the supernatant of BAEC stimulated with NaP (100 μM, 4 h) and pretreated with vehicle, OME 100 μg/mL, or Na 2 S (100 μM) 30 min before challenge. Data are reported as nM and relative DCF fluorescence, respectively, and expressed as means ± SEM.

Article Snippet: Bovine aortic endothelial cells (BAECs) were purchased from iXCells Biotechnologies (Cat# 10BO-001) and were cultured in Modified Dulbecco's Medium (DMEM) supplemented with 2 mmol/L glutamine, 10% heat-inactivated fetal bovine serum, and 50 U/mL penicillin/streptomycin.

Techniques: Fluorescence